Yes – but you need to rename it or place it as uni-bios.rom inside a modified neogeo.zip . Some custom builds exist. However, standard FBNeo expects the official SNK BIOS first.

If you’re setting up FinalBurn Neo today, treat neogeo.zip with respect. Place it correctly, keep it updated, and it will faithfully serve up the glory days of SNK—one credit at a time.

Most systems like RetroArch or RetroPie need it in the /ROMs/neogeo/ folder or the /BIOS/fbneo/ directory.