Primer3 0.4.0 -

| Feature | Primer3 1.1.4 | Primer3 2.3.6 | | |---------|---------------|---------------|-------------------| | Tm model | Breslauer 86 | SantaLucia 98 | SantaLucia 98 | | Salt correction | 1M only | Owczarzy 04 | Owczarzy 04 | | Degenerate primers | No | Yes | Yes | | Multiplex penalty | No | Yes | Partial (output only) | | API stability | Poor | Good | Excellent | | Thread safety | No | No | Yes | | Memory footprint | Low | Medium | Very low |

v0.4.0 introduced more robust task handling via the PRIMER_TASK flag, allowing the engine to act as a multi-purpose tool: primer3 0.4.0

To install via package managers: